Antisense oligonucleotides (ASOs) are small, single-stranded molecules that can suppress the expression of targeted genes and thereby achieve therapeutic effects. However, many ASO-based drug ...

Researchers in China have developed a new method that allows them to insert long stretches of DNA into a genome. The process, named GRAND editing, is one of a handful of new systems aimed at using two ...

Researchers from Tokyo Medical and Dental University (TMDU) and Ionis Pharmaceuticals, USA, report a modification wherein replacing the RNA strand of a heteroduplex oligonucleotide with DNA may ...

CRISPR-Cas9 has been editing the human genome for the past decade. Although the types of edits have varied, an unsolved challenge has been the integration of large pieces of DNA without ...

Figure 1. Schematic illustration of the spontaneous Hoogsteen pairing-based strand displacement reaction between one homo-dimolecular GQ target and dual G-rich invading probes at room temperature, ...

DNA, or deoxyribonucleic acid, is the hereditary material found in nearly all living organisms. It carries the genetic instructions for the development, functioning, growth, and reproduction of all ...

The pegRNA binds to a matching sequence on the target DNA strand. The Cas9 nickase cuts the unbound complementary strand, creating a flap. The dangling DNA flap binds to a matching sequence on the ...